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Coenzyme B12 Enzymes Part B
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ISBN: 9780323955577
Published: 2022
Publisher: Academic Press
Language: English
Format: Hardback
Format: 9×6
Published: 2022
Publisher: Academic Press
Language: English
Format: Hardback
Format: 9×6
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<p><i>Coenzyme B12 Enzymes, Part B, Volume 169</i> in the <i>Methods in Enzymology</i> series, highlights new advances in the field, with this new volume presenting interesting chapters on Structural characterization of cobalamin-dependent radical SAM methylases, Purification and characterization of sequential cobalamin-dependent radical SAM methylases ThnK and TokK in -lactam antibiotic biosynthesis, Characterization of the cobalamin-dependent radical S-adenosyl-L-methionine enzyme C-methyltransferase Fom3 in fosfomycin biosynthesis, Studies of OxsB and GenK, two B12-dependent radical SAM enzymes involved in natural product biosynthesis, Purification and structural elucidation of the cobalamin-dependent radical SAM enzyme OxsB, and more.</p> <p>Other chapters discuss Methods for studying the mechanisms of B12 enzymes, Computational investigations of B12 dependent enzymatic reactions, Using kinetic isotope effects to probe the mechanisms of adenosylcobalamin-dependent enzymes, Steady-state and pre-steady state kinetic analysis of ornithine 4,5-aminomutase, and more.</p>
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