Radiation Oncology and Radiotherapy, Part A

<p><i>Radiation Oncology and Radiotherapy, Part A, Volume 172 </i>in the <i>Methods in Cell Biology</i> series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more.</p> <p>Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2a phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest.</p>